notch signaling antagonist  (Deltex Medical)

Journal:

Article Title: Modulation of Notch Signaling Elicits Signature Tumors and Inhibits Hras1-Induced Oncogenesis in the Mouse Mammary Epithelium

doi:

Figure Lengend Snippet: Nonregressing hN1IC-dependent tumors. Nonregressing hN1IC-dependent papillary invasive adenocarcinomas with low-grade nuclei and necrotic areas. A: Invasion of adipose tissue by a papillary adenocarcinoma. B: An invasive tumor (Inv) is found adjacent to a benign papillary lesion in a terminal breast duct (arrow). DCIS is also visible in this section and is indicated by an asterisk. C: Invasive tumor with evident papillary structure composed of (enlargement in D) a uniform population of cancer cells with low-grade nuclei, sharp nuclear borders, and inconspicuous nucleoli. Regressing papillary hN1IC neoplasias are more sensitive to apoptosis than the invasive tumors. E and F: Apoptotic cells identified by TUNEL analysis (brown staining) in hN1IC lactation-dependent tumors from animals sacrificed either during lactation (E) or during involution (F). Note the increased number of the TUNEL-positive cells in F, as compared to E, consistent with the notion that tumors induced during lactation retain the ability to respond to apoptotic signals during involution. G and H: TUNEL analysis in nonregressing tumors during lactation and involution, respectively. The scarcity of apoptotic nuclei, as compared to that of the regressing tumors shown in E and F indicates that these lesions are resistant to the apoptotic stimuli encountered during involution. Original magnifications: ×4 (B); ×10 (A, C); ×60 (D).

Article Snippet: However, terminal differentiation of the mammary epithelium during lactation could proceed normally. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 6 caption a7 The expression of the Notch signal antagonist Deltex inhibits apoptosis during involution.

Techniques: TUNEL Assay, Staining

Journal:

Article Title: Modulation of Notch Signaling Elicits Signature Tumors and Inhibits Hras1-Induced Oncogenesis in the Mouse Mammary Epithelium

doi:

Figure Lengend Snippet: The expression of the Notch signal antagonist Deltex inhibits apoptosis during involution. The ratio of apoptotic versus total number of cells was evaluated by hematoxylin staining and TUNEL in animals at day 2 of involution. Cells were counted in groups of three to five ducts with each group deriving from an individual gland. A total of three glands, each from a different mouse, are included in this analysis. Top panels show an analysis based on the identification of apoptotic figures (examples are indicated by arrows) revealed by hematoxylin staining, and the bottom panels identify apoptosis by TUNEL (examples are indicated by arrowheads) in wild-type (WT) and MMTV-hDTX1 (hDTX1) animals at day 2 of involution. Both methods indicate that the expression of the Notch antagonist Deltex inhibits apoptosis during involution, indicating an opposite phenotype seen in MMTV-hN1IC(hN1IC) in which involution-dependent apoptosis seems intact and may even be elevated. The average values of the apoptotic cells determined by the two methods are also shown in the graphs.

Article Snippet: However, terminal differentiation of the mammary epithelium during lactation could proceed normally. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 6 caption a7 The expression of the Notch signal antagonist Deltex inhibits apoptosis during involution.

Techniques: Expressing, Staining, TUNEL Assay